P450 Assays
Cytochrome P450, a protein found in the human liver, is responsible for the metabolism of a significant number of therapeutic agents and substrates. In a P450 assay experiment, researchers make modifications to a drug, and then evaluate the potency and selectivity of the drug.
Using the Nanostream LD System, you can rapidly conduct P450 assays. The system employs conventional, label-free substrates and offers the capability to detect less than 10% conversion of substrate to products. Thus, the system yields data with higher specificity than plate readers and allows you to achieve higher throughput compared to LC/MS.
IC50 was determined to be 0.12 µM after 30 µM testosterone was incubated in the presence of Cytochrome P450 3A4 and a serial dilution of ketoconazole for one hour at 37°C and analyzed using the Nanostream LD System.