Glucuronidation Assays
Glucuronidation is an important phase 2 metabolism process by which a sugar moiety is enzymatically conjugated to a compound of interest via -OH, -NH2, -SH or -COOH functional groups. The resulting conjugate or glucuronide is more hydrophilic than the original compound, which facilitates elimination through excretion.
Using the Nanostream LD System, you can develop a simple and convenient assay for the study of drug-drug interactions related to UGTs. The system significantly reduces the analysis time for assay development and solvent used for the experiment.
Simultneous separation of product from the substrate and matrix components for 24 samples from incubated hepatocytes.